|
KMID : 0903519970400050369
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1997 Volume.40 No. 5 p.369 ~ p.374
|
|
|
Isolation and Characterization of Bacillus sp . P16 Producing Extracellular Chitosanase
|
|
Jung Mi-Ra
Park Ro-Dong
Jo Yoo-Young
Chi Yeon-Tae
|
|
|
Abstract
|
|
|
|
An endochitosanase-producing bacterium was isolated from soil and identified as a strain of Bacillus sp. The isolate was gram positive, rod shape (0.4-0.6×1.6-2.2 ㎛), endospore-forming, catalase positive, and mobility positive, and grown at pH 4.5-11.0 and upto 42℃ in the medium containing 2% NaCl. RAPD analysis of the DNA purified from the strain was also performed, and the chitosanase-producing strain was named as Bacillus sp. P16. The culture supernatant of the strain showed strong liquefaction activity and rapidly decreased viscosity of chitosan solution. By TLC and HPLC, chitooligosaccharides of DP 2-7 were separated and identified from the enzyme hydrolyzates of chitosan. The chitosanase from Bacillus sp. P16 was thus regarded as an endo-splitting type.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|